Lu, Maryia, Tay, Li Wei Rachel, He, Jingquan, Du, Guangwei, Maryia Lu, Li Wei Rachel Tay, Jingquan He, Guangwei Du
Abstract
Phospholipids are important signaling molecules that regulate cell proliferation, death, migration, and metabolism. Many phospholipid signaling cascades are altered in breast cancer. To understand the functions of phospholipid signaling molecules, genetically encoded phospholipid biosensors have been developed to monitor their spatiotemporal dynamics. Compared to other phospholipids, much less is known about the subcellular production and cellular functions of phosphatidic acid (PA), partially due to the lack of a specific and sensitive PA biosensor in the past. This chapter describes the use of a newly developed PA biosensor, PASS, in two applications: regular fluorescent microscopy and fluorescence lifetime imaging microscopy-Förster/fluorescence resonance energy transfer (FLIM-FRET). These protocols can be also used with other phospholipid biosensors.
Pharmacology, Toxicology and Pharmaceutical Science
2
11%
Agricultural and Biological Sciences
2
11%
Engineering
2
11%
Immunology and Microbiology
1
5%
Other
2
11%
Unknown
4
21%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 February 2016.
All research outputs
#13,221,334
of 22,842,950 outputs
Outputs from Methods in molecular biology
#3,465
of 13,127 outputs
Outputs of similar age
#184,634
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#328
of 1,470 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,571 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.
