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Breast Cancer

Overview of attention for book
Breast Cancer
Springer New York

Table of Contents

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    Book Overview
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    Chapter 1 Basic Histopathological Methods and Breast Lesion Types for Research.
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    Chapter 2 Clinical Applications for Immunohistochemistry of Breast Lesions
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    Chapter 3 Immunohistochemistry for Triple-Negative Breast Cancer.
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    Chapter 4 In Situ Hybridization of Breast Cancer Markers.
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    Chapter 5 Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).
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    Chapter 6 Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).
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    Chapter 7 Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.
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    Chapter 8 Pyrosequencing Analysis for Breast Cancer DNA Methylome.
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    Chapter 9 Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).
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    Chapter 10 Breast Cancer Stem Cell Isolation.
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    Chapter 11 Cellular Apoptosis Assay of Breast Cancer.
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    Chapter 12 Assessment of Matrix Metalloproteinases by Gelatin Zymography.
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    Chapter 13 Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay
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    Chapter 14 Determination of Breast Cancer Cell Migratory Ability.
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    Chapter 15 A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
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    Chapter 16 Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.
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    Chapter 17 A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation
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    Chapter 18 Analysis of Invadopodia Formation in Breast Cancer Cells.
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    Chapter 19 Patient-Derived Tumor Xenograft Models of Breast Cancer.
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    Chapter 20 Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors.
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    Chapter 21 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method
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    Chapter 22 Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model
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    Chapter 23 Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.
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    Chapter 24 Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.
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    Chapter 25 Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.
Attention for Chapter 9: Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).
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  • Good Attention Score compared to outputs of the same age and source (68th percentile)

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Chapter title
Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).
Chapter number 9
Book title
Breast Cancer
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3444-7_9
Pubmed ID
Book ISBNs
978-1-4939-3442-3, 978-1-4939-3444-7
Authors

Tulley, Shaun, Zhao, Qiang, Dong, Huan, Pearl, Michael L, Chen, Wen-Tien, Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, Wen-Tien Chen, Pearl, Michael L.

Abstract

The ability to capture, enrich, and propagate circulating cancer cells/circulating tumor cells (CTCs) for downstream analyses such as ex vivo drug-sensitivity testing of short-term cultures of CTCs, single cell sorting of CTCs by fluorescence activated cell sorting (FACS), animal injection tumor and/or metastasis formation studies, next generation sequencing (NGS), gene expression profiling, gene copy number determination, and epigenomic analyses is of high priority and of immense importance to both the basic research and translational/clinical research communities. Vitatex Inc.'s functional cell separation technology, constructed as Vita-Assay™ (AG6W, AN6W, AR6W) culture plates, is based on the preferential adhesion of invasive rare blood cells of tissue origin to a tissue or tumor microenvironment mimic-the so-called cell adhesion matrix (CAM), which has a demonstrated ability to enrich viable CTCs from blood up to one-million fold.The CAM-scaffold allows for the functional capture and identification of invasive CTCs (iCTCs) including invasive tumor progenitor (TP) cells from cancer-patients' blood. CAM-captured CTCs are capable of ingesting the CAM (CAM+) itself. Green and red fluorescent versions of Vita-Assay™ (AG6W and AR6W) allow for direct visualization of CAM-uptake by cancer cells. Vita-Assay™ CAM-enrichment has allowed for sensitive multiplex flow cytometric and microscopic detection of iCTCs from patients with cancers of the breast, ovary, prostate, pancreas, colorectum, and lung; it has also been successfully utilized for ex vivo drug-sensitivity testing of ovarian-cancer patient CTCs. The CAM enrichment method is equally suitable for the separation of iCTCs and TP cells in ascites and pleural fluid.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 23%
Student > Ph. D. Student 5 16%
Student > Bachelor 4 13%
Student > Doctoral Student 4 13%
Student > Master 2 6%
Other 1 3%
Unknown 8 26%
Readers by discipline Count As %
Medicine and Dentistry 8 26%
Agricultural and Biological Sciences 5 16%
Biochemistry, Genetics and Molecular Biology 4 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Chemistry 1 3%
Other 1 3%
Unknown 11 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 January 2016.
All research outputs
#14,246,461
of 22,842,950 outputs
Outputs from Methods in molecular biology
#4,193
of 13,127 outputs
Outputs of similar age
#206,001
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#417
of 1,470 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,571 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.