| Chapter title |
Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation
|
|---|---|
| Chapter number | 9 |
| Book title |
Programmed Necrosis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8754-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
|
| Authors |
Roshan J. Thapa, Shoko Nogusa, Siddharth Balachandran, Thapa, Roshan J., Nogusa, Shoko, Balachandran, Siddharth |
| Abstract |
In multicellular organisms, regulated cell death plays a vital role in development, adult tissue homeostasis, and clearance of damaged or infected cells. Necroptosis is one such form of regulated cell death, characterized by its reliance on receptor-interacting protein kinase 3 (RIPK3). Once activated, RIPK3 nucleates a protein complex, termed the "necrosome," which includes the adaptors RIPK1 and FADD, and the effector protein MLKL. From the necrosome, RIPK3 phosphorylates MLKL to drive necroptosis, and can also induce RIPK1/FADD-mediated apoptosis, via caspase-8. Assembly of the necrosome thus serves as a useful readout of RIPK3 activation. In this chapter, we describe molecular methods for examining necrosome activation in response to the cytokines TNF-α, IFN-β, and IFN-γ, and upon infection with influenza A virus (IAV). |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 50% |
| Professor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Master | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Immunology and Microbiology | 2 | 33% |
| Agricultural and Biological Sciences | 1 | 17% |