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Mendeley readers
| Chapter title |
Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules
|
|---|---|
| Chapter number | 2 |
| Book title |
Programmed Necrosis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8754-2_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
|
| Authors |
David Mark Moquin, Francis Ka-Ming Chan |
| Abstract |
Over the recent years, genome-wide RNA interference (RNAi) library screens have been instrumental in the identification of key regulators of various biological pathways. The prolific use of this technique is attributed to its amenability to a high-throughput format. Here, we present the step-by-step method to conduct a siRNA screen to identify genes involved in necroptosis, a nonapoptotic form of proinflammatory cell death. The method described here uses MTS cell proliferation assay to measure necroptosis, which is compatible with high-throughput format screening on multiwell microtiter plates. This ensures that the screen can be performed in a timely and efficient manner. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 50% |
| Student > Master | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |