Chapter title |
Analyzing Necroptosis Using an RIPK1 Kinase Inactive Mouse Model of TNF Shock
|
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Chapter number | 12 |
Book title |
Programmed Necrosis
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8754-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
|
Authors |
Matija Zelic, Michelle A. Kelliher |
Abstract |
The serine/threonine kinase RIPK1 has numerous biological and pathological functions, mediating prosurvival as well as prodeath apoptotic and necroptotic signaling pathways downstream of various receptors, including death receptors and Toll-like receptors (TLRs). RIPK1 has been implicated in various diseases, including ischemia-reperfusion injury and inflammatory bowel disease (IBD). The recent generation of RIPK1 kinase inactive mice has enabled us to genetically interrogate the role of RIPK1 kinase-mediated necroptosis in disease models. Here, we describe procedures utilizing kinase inactive Ripk1D138N/D138N mice to analyze necroptosis induction in vitro in bone-marrow derived macrophages (BMDMs) and in vivo in a murine model of TNF-induced shock. |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Unspecified | 1 | 25% |
Student > Ph. D. Student | 1 | 25% |
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Unknown | 1 | 25% |
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Unspecified | 1 | 25% |
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Unknown | 1 | 25% |