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Chapter title |
Detection of MLKL Oligomerization During Programmed Necrosis
|
---|---|
Chapter number | 8 |
Book title |
Programmed Necrosis
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8754-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
|
Authors |
Zhenyu Cai, Zheng-Gang Liu |
Abstract |
Programmed necrosis, also known as necroptosis, is a form of regulated necrotic cell death that is mediated by receptor-interacting protein kinases RIP1 (or RIPK1), RIP3 (or RIPK3), and the mixed lineage kinase domain-like protein, MLKL. Following the induction of programmed necrosis, MLKL is phosphorylated by RIP3 and oligomerizes and then the protein translocates to cell plasma membrane in order to execute programmed necrosis. Here, we describe a detailed protocol to detect MLKL oligomerization in necroptotic cells by Western blotting analysis under nonreducing condition. Therefore, we established the method to detect the activation of programmed necrotic pathway. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Unspecified | 2 | 14% |
Professor | 2 | 14% |
Student > Bachelor | 2 | 14% |
Student > Master | 2 | 14% |
Other | 2 | 14% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 14% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 2 | 14% |
Unknown | 4 | 29% |