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Mendeley readers
| Chapter title |
Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling
|
|---|---|
| Chapter number | 15 |
| Book title |
Programmed Necrosis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8754-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
|
| Authors |
Joshua D. Webster, Margaret Solon, Susan Haller, Kim Newton |
| Abstract |
Activation of the kinase RIPK3 (receptor interacting protein kinase 3) is a hallmark of cells dying by necroptosis. RIPK3 phosphorylates both itself and the pseudokinase MLKL (mixed lineage kinase-like) resulting in MLKL translocation to membranes and cell lysis. Antibodies recognizing RIPK3 autophosphorylation or the RIPK3-dependent phosphorylation sites on MLKL have therefore been used to monitor necroptosis induction. Here we describe immunohistochemical labeling for autophosphorylated mouse RIPK3 as a means of detecting cells undergoing necroptosis in mouse tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Ph. D. Student | 2 | 17% |
| Student > Master | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Neuroscience | 1 | 8% |
| Unknown | 6 | 50% |