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Chapter title |
Analysis of CYLD Proteolysis by CASPASE 8 in Bone Marrow-Derived Macrophages
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Chapter number | 18 |
Book title |
Programmed Necrosis
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8754-2_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8753-5, 978-1-4939-8754-2
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Authors |
Diana Legarda, Adrian T. Ting |
Abstract |
Previous studies have demonstrated that CASPASE 8 can generate a prosurvival signal by inhibiting necroptosis via the cleavage of the deubiquitinating enzyme CYLD. Cleavage of CYLD at D215 results in the generation of a 25 kD N-terminal fragment and degradation of the C-terminal fragment containing the catalytic domain. Since CYLD is required for TNF-induced necroptosis, its proteolysis is necessary and sufficient to suppress necroptosis and generate a survival signal. Here we describe how to visualize CYLD proteolysis by western blot analysis, as a measure of CASPASE 8 activity and inhibition of necroptosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Immunology and Microbiology | 1 | 33% |
Unknown | 1 | 33% |