Chapter title |
A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates
|
---|---|
Chapter number | 5 |
Book title |
Lipases and Phospholipases
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8672-9_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8671-2, 978-1-4939-8672-9
|
Authors |
Meddy El Alaoui, Laurent Soulère, Alexandre Noiriel, Priscila Sutto-Ortiz, Lucie Grand, Florence Popowycz, Jorge Alberto Rodríguez-González, Yves Queneau, Abdelkarim Abousalham, Alaoui, Meddy El, Soulère, Laurent, Noiriel, Alexandre, Sutto-Ortiz, Priscila, Grand, Lucie, Popowycz, Florence, Rodríguez-González, Jorge Alberto, Queneau, Yves, Abousalham, Abdelkarim |
Abstract |
To date, several sensitive methods, based on radiolabeled elements or sterically hindered fluorochrome groups, are usually employed to screen lipase and phospholipase A (PLA) activities. Here, a new ultraviolet spectrophotometric assay for lipase or PLA was developed using natural triglycerides or synthetic glycerophosphatidylcholines containing α-eleostearic acid (9Z, 11E, 13E-octadecatrienoic acid) purified from Aleurites fordii seed oil. The conjugated triene present in α-eleostearic acid constitutes an intrinsic chromophore and consequently confers strong UV absorption properties of this free fatty acid as well as of lipid substrates harboring it. The substrate was coated into the wells of a microplate, and the lipolytic activities were measured by the absorbance increase at 272 nm due to the transition of α-eleostearic acid moiety from the adsorbed to the soluble state. This continuous assay is compatible with a high-throughput screening method and can be applied specifically to the screening of new potential lipase, PLA1 and PLA2 inhibitors. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 31% |
Researcher | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Professor | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Agricultural and Biological Sciences | 3 | 23% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 5 | 38% |