Chapter title |
Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases
|
---|---|
Chapter number | 14 |
Book title |
Lipases and Phospholipases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8672-9_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8671-2, 978-1-4939-8672-9
|
Authors |
Del Monte-Martínez, Alberto, Cutiño-Avila, Bessy V, González-Bacerio, Jorge, Alberto del Monte-Martínez, Bessy V. Cutiño-Avila, Jorge González-Bacerio, Monte-Martínez, Alberto del, Cutiño-Avila, Bessy V. |
Abstract |
Immobilization of lipases and phospholipases, mainly on water-insoluble carriers, helps in their economic reusing and in the development of continuous bioprocesses. Design of efficient lipase and phospholipase-immobilized systems is rather a difficult task. A lot of research work has been done in order to optimize immobilization techniques and procedures and to develop efficient immobilized systems. We conceived a new strategy for the rational design of immobilized derivatives (RDID) in favor of the successful synthesis of optimal lipase and phospholipase-immobilized derivatives, aiming the prediction of the immobilized derivative's functionality and the optimization of load studies. The RDID strategy begins with the knowledge of structural and functional features of synthesis components (protein and carrier) and the practical goal of the immobilized product. The RDID strategy was implemented in a software named RDID1.0. The employment of RDID allows selecting the most appropriate way to prepare immobilized derivatives more efficient in enzymatic bioconversion processes and racemic mixture resolution. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 3 | 60% |
Student > Ph. D. Student | 1 | 20% |
Professor | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 60% |
Engineering | 1 | 20% |
Unknown | 1 | 20% |