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Lipases and Phospholipases

Overview of attention for book
Cover of 'Lipases and Phospholipases'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Lipases: An Overview
  3. Altmetric Badge
    Chapter 2 Carbohydrate Esterases: An Overview
  4. Altmetric Badge
    Chapter 3 Phospholipases: An Overview
  5. Altmetric Badge
    Chapter 4 Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
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    Chapter 5 A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates
  7. Altmetric Badge
    Chapter 6 Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline
  8. Altmetric Badge
    Chapter 7 Lipase and Phospholipase Activity Methods for Marine Organisms
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    Chapter 8 Functional Expression of Plant Lipases: The Case of CpLip1 from Carica papaya
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    Chapter 9 Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2
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    Chapter 10 Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies
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    Chapter 11 Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review
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    Chapter 12 Solid-State Fermentation as an Economic Production Method of Lipases
  14. Altmetric Badge
    Chapter 13 Synthetic Biology to Improve the Production of Lipases and Esterases (Review)
  15. Altmetric Badge
    Chapter 14 Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases
  16. Altmetric Badge
    Chapter 15 Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides
  17. Altmetric Badge
    Chapter 16 Targeting Phospholipase D Genetically and Pharmacologically for Studying Leukocyte Function
  18. Altmetric Badge
    Chapter 17 Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale
  19. Altmetric Badge
    Chapter 18 Lipase-Catalyzed Acetylation and Esterification of Bile Acids
  20. Altmetric Badge
    Chapter 19 Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents
  21. Altmetric Badge
    Chapter 20 Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases
  22. Altmetric Badge
    Chapter 21 Lipases as Biocatalyst for Biodiesel Production
  23. Altmetric Badge
    Chapter 22 Lipase, Phospholipase, and Esterase Biosensors (Review)
Attention for Chapter 4: Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
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Chapter title
Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
Chapter number 4
Book title
Lipases and Phospholipases
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8672-9_4
Pubmed ID
30109647
Book ISBNs
978-1-4939-8671-2, 978-1-4939-8672-9
Authors

Dolores Reyes-Duarte, Cristina Coscolín, Mónica Martínez-Martínez, Manuel Ferrer, Humberto García-Arellano, Reyes-Duarte, Dolores, Coscolín, Cristina, Martínez-Martínez, Mónica, Ferrer, Manuel, García-Arellano, Humberto

Abstract

Functional screens have been extensively used for searching native enzymes or mutant variants in clone libraries. Esterases and lipases are the most retrieved enzymes, because they are within the more demanded industrial enzymes and because a number of simple and generic screening methods can be applied for their screen. Here, we describe the use of a generic pH indicator assay protocol which unambiguously allows detecting in high-throughput manner esterase and lipase activity and quantifying specific activities using an ester concentration above 0.5 mM. The described method is simple and generic to allow the selection of esterases and lipases targeting desired esters.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 21%
Student > Ph. D. Student 4 21%
Student > Doctoral Student 2 11%
Researcher 2 11%
Student > Master 2 11%
Other 1 5%
Unknown 4 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 47%
Agricultural and Biological Sciences 4 21%
Chemistry 1 5%
Unknown 5 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2018.
All research outputs
#18,909,315
of 24,093,053 outputs
Outputs from Methods in molecular biology
#7,740
of 13,601 outputs
Outputs of similar age
#242,603
of 334,094 outputs
Outputs of similar age from Methods in molecular biology
#139
of 246 outputs
Altmetric has tracked 24,093,053 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,601 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,094 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 246 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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