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Lipases and Phospholipases

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Cover of 'Lipases and Phospholipases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Lipases: An Overview
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    Chapter 2 Carbohydrate Esterases: An Overview
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    Chapter 3 Phospholipases: An Overview
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    Chapter 4 Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
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    Chapter 5 A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates
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    Chapter 6 Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline
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    Chapter 7 Lipase and Phospholipase Activity Methods for Marine Organisms
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    Chapter 8 Functional Expression of Plant Lipases: The Case of CpLip1 from Carica papaya
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    Chapter 9 Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2
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    Chapter 10 Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies
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    Chapter 11 Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review
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    Chapter 12 Solid-State Fermentation as an Economic Production Method of Lipases
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    Chapter 13 Synthetic Biology to Improve the Production of Lipases and Esterases (Review)
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    Chapter 14 Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases
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    Chapter 15 Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides
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    Chapter 16 Targeting Phospholipase D Genetically and Pharmacologically for Studying Leukocyte Function
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    Chapter 17 Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale
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    Chapter 18 Lipase-Catalyzed Acetylation and Esterification of Bile Acids
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    Chapter 19 Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents
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    Chapter 20 Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases
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    Chapter 21 Lipases as Biocatalyst for Biodiesel Production
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    Chapter 22 Lipase, Phospholipase, and Esterase Biosensors (Review)
Attention for Chapter 12: Solid-State Fermentation as an Economic Production Method of Lipases
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Chapter title
Solid-State Fermentation as an Economic Production Method of Lipases
Chapter number 12
Book title
Lipases and Phospholipases
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8672-9_12
Pubmed ID
Book ISBNs
978-1-4939-8671-2, 978-1-4939-8672-9
Authors

Doddy Denise Ojeda-Hernández, Ricardo Cosío-Cuadros, Georgina Sandoval, Jorge Alberto Rodríguez-González, Juan Carlos Mateos-Díaz, Ojeda-Hernández, Doddy Denise, Cosío-Cuadros, Ricardo, Sandoval, Georgina, Rodríguez-González, Jorge Alberto, Mateos-Díaz, Juan Carlos

Abstract

Solid-state fermentation (SSF) has been largely employed during the last three decades to produce different biomolecules of industrial interest, particularly enzymes. Through the use of agroindustrial wastes as SSF substrates, an economic process of lipases production can be achieved. In this chapter we describe a comprehensive SSF method for producing an economical preparation of Rhizomucor miehei lipase, employing sugarcane bagasse and used vegetal oil as substrates. To demonstrate the usefulness of the lipase produced by this method, we utilized directly the dried fermented solid, as a heterogeneous biocatalyst for the ethanolysis of different fats and oils. Final ethyl ester conversions (>90%, 24 h) were similar with those obtained using a commercial immobilized Rhizomucor miehei lipase at our best conditions. In this work we demonstrated that SSF is an easy and economical method for the production of lipases that can be used directly as heterogeneous biocatalysts for biodiesel production, employing low-cost feedstocks.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 35%
Student > Ph. D. Student 3 18%
Professor 2 12%
Unspecified 1 6%
Student > Master 1 6%
Other 0 0%
Unknown 4 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 24%
Agricultural and Biological Sciences 3 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Unspecified 1 6%
Nursing and Health Professions 1 6%
Other 3 18%
Unknown 4 24%