Chapter title |
Lipase and Phospholipase Activity Methods for Marine Organisms
|
---|---|
Chapter number | 7 |
Book title |
Lipases and Phospholipases
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8672-9_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8671-2, 978-1-4939-8672-9
|
Authors |
H. Nolasco-Soria, F. Moyano-López, F. Vega-Villasante, Alberto del Monte-Martínez, D. Espinosa-Chaurand, E. Gisbert, H. R. Nolasco-Alzaga, Nolasco-Soria, H., Moyano-López, F., Vega-Villasante, F., Monte-Martínez, Alberto del, Espinosa-Chaurand, D., Gisbert, E., Nolasco-Alzaga, H. R. |
Abstract |
Lipases are very important enzymes having a role in fat digestion and lipid metabolism in marine animals, plants, and microorganisms. The methods for measuring lipase and phospholipase activity have been applied in several studies; however, considering that lipases are water-soluble molecules and their substrates are generally water-insoluble molecules, several steps are required for measuring their digestion products. After a general review of the main type of methods used in marine lipase studies, and experimental procedures, a proposal of new or improved methods is described in order to facilitate the lipase activity measurements in marine organisms. |
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