Chapter title |
Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
|
---|---|
Chapter number | 11 |
Book title |
Baculovirus and Insect Cell Expression Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Christine M. Thompson, Johnny Montes, Marc G. Aucoin, Amine A. Kamen, Thompson, Christine M., Montes, Johnny, Aucoin, Marc G., Kamen, Amine A. |
Abstract |
The production of recombinant proteins using the baculovirus expression vector system (BEVS) in large-scale agitated bioreactors is discussed in this chapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Mexico | 1 | 2% |
France | 1 | 2% |
Australia | 1 | 2% |
Unknown | 43 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 24% |
Student > Master | 8 | 17% |
Student > Ph. D. Student | 7 | 15% |
Student > Bachelor | 6 | 13% |
Other | 2 | 4% |
Other | 3 | 7% |
Unknown | 9 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 35% |
Biochemistry, Genetics and Molecular Biology | 13 | 28% |
Chemical Engineering | 2 | 4% |
Medicine and Dentistry | 2 | 4% |
Engineering | 2 | 4% |
Other | 0 | 0% |
Unknown | 11 | 24% |