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Baculovirus and Insect Cell Expression Protocols

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Cover of 'Baculovirus and Insect Cell Expression Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior
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    Chapter 2 Introduction to Baculovirus Molecular Biology
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    Chapter 3 Baculovirus Transfer Vectors
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    Chapter 4 Recombinant Baculovirus Isolation
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    Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
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    Chapter 6 Available Lepidopteran Insect Cell Lines.
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    Chapter 7 Lepidopteran Insect Cell Line Isolation from Insect Tissue
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    Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines
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    Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
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    Chapter 10 Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System
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    Chapter 11 Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
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    Chapter 12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
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    Chapter 13 Protein Production with Recombinant Baculoviruses in Lepidopteran Larvae
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    Chapter 14 Production of Virus-Like Particles for Vaccination
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    Chapter 15 Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
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    Chapter 16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
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    Chapter 17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression.
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    Chapter 18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
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    Chapter 19 Introduction to the Use of Baculoviruses as Biological Insecticides
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    Chapter 20 Baculovirus Insecticide Production in Insect Larvae
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    Chapter 21 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
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    Chapter 22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
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    Chapter 23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production
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    Chapter 24 Gene Silencing in Insect Cells Using RNAi
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    Chapter 25 Using the Baculovirus/Insect Cell System to Study Apoptosis
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    Chapter 26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells
Attention for Chapter 14: Production of Virus-Like Particles for Vaccination
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Chapter title
Production of Virus-Like Particles for Vaccination
Chapter number 14
Book title
Baculovirus and Insect Cell Expression Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3043-2_14
Pubmed ID
Book ISBNs
978-1-4939-3042-5, 978-1-4939-3043-2
Authors

Christine M. Thompson, Marc G. Aucoin, Amine A. Kamen, Thompson, Christine M., Aucoin, Marc G., Kamen, Amine A.

Abstract

The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as vaccine candidates, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. The use of host insect cells allows mass production of VLPs in a proven scalable system.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 35%
Researcher 4 20%
Student > Ph. D. Student 3 15%
Student > Doctoral Student 2 10%
Student > Bachelor 2 10%
Other 3 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 25%
Biochemistry, Genetics and Molecular Biology 5 25%
Immunology and Microbiology 4 20%
Chemical Engineering 3 15%
Unspecified 1 5%
Other 2 10%