Chapter title |
Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
|
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Chapter number | 12 |
Book title |
Baculovirus and Insect Cell Expression Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Sue H. Kadwell, Laurie K. Overton, Kadwell, Sue H., Overton, Laurie K. |
Abstract |
Many types of disposable bioreactors for protein expression in insect and mammalian cells are now available. They differ in design, capacity, and sensor options, with many selections available for either rocking platform, orbitally shaken, pneumatically mixed, or stirred-tank bioreactors lined with an integral disposable bag (Shukla and Gottschalk, Trends Biotechnol 31(3):147-154, 2013). WAVE Bioreactors™ were among the first disposable systems to be developed (Singh, Cytotechnology 30:149-158, 1999). Since their commercialization in 1999, Wave Bioreactors have become routinely used in many laboratories due to their ease of operation, limited utility requirements, and protein expression levels comparability to traditional stirred-tank bioreactors. Wave Bioreactors are designed to use a presterilized Cellbag™, which is attached to a rocking platform and inflated with filtered air provided by the bioreactor unit. The Cellbag can be filled with medium and cells and maintained at a set temperature. The rocking motion, which is adjusted through angle and rock speed settings, provides mixing of oxygen (and CO2, which is used to control pH in mammalian cell cultures) from the headspace created in the inflated Cellbag with the cell culture medium and cells. This rocking motion can be adjusted to prevent cell shear damage. Dissolved oxygen and pH can be monitored during scale-up, and samples can be easily removed to monitor other parameters. Insect and mammalian cells grow very well in Wave Bioreactors (Shukla and Gottschalk, Trends Biotechnol 31(3):147-154, 2013). Combining Wave Bioreactor cell growth capabilities with recombinant baculoviruses engineered for insect or mammalian cell expression has proven to be a powerful tool for rapid production of a wide range of proteins. |
Mendeley readers
Geographical breakdown
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Unknown | 18 | 100% |
Demographic breakdown
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Researcher | 6 | 33% |
Other | 3 | 17% |
Student > Bachelor | 1 | 6% |
Lecturer | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 28% |
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Unknown | 6 | 33% |