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Mendeley readers
| Chapter title |
Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System
|
|---|---|
| Chapter number | 10 |
| Book title |
Baculovirus and Insect Cell Expression Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3043-2_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
| Authors |
Jian-Ping Yang, Yang, Jian-Ping |
| Abstract |
Numerous technological improvements, including progress in vector design, simplification of virus isolation techniques, and advancements in molecular biology and cell culture technologies, have greatly facilitated the use of the baculovirus-insect cell system for routine production of recombinant proteins. This chapter outlines the basic techniques for small-scale protein production using the Baculovirus Expression Vector System (BEVS), including protocols for titer estimation in 96-well plates, expression optimization in 24-well plates, and recombinant protein expression from adherent and suspension cultures in six-well plates and in 50 mL insect cell cultures. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 24% |
| Researcher | 4 | 24% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Master | 3 | 18% |
| Other | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 47% |
| Agricultural and Biological Sciences | 5 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Unknown | 2 | 12% |