Chapter title |
Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
|
---|---|
Chapter number | 5 |
Book title |
Baculovirus and Insect Cell Expression Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Fornwald, James A, Lu, Quinn, Boyce, Frederick M, Ames, Robert S, James A. Fornwald, Quinn Lu, Frederick M. Boyce, Robert S. Ames, Fornwald, James A., Boyce, Frederick M., Ames, Robert S. |
Abstract |
BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. BacMams have become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide. Construction of transfer vectors is straightforward using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV. |
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