Chapter title |
Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
|
---|---|
Chapter number | 21 |
Book title |
Baculovirus and Insect Cell Expression Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Holly J. R. Popham, Mark R. Ellersieck, Huarong Li, Bryony C. Bonning, Popham, Holly J. R., Ellersieck, Mark R., Li, Huarong, Bonning, Bryony C. |
Abstract |
A considerable amount of work has been undertaken to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50 % of infected larvae (LC50 or LD50), median survival of larvae infected (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and the corresponding data analyses for assessment of the insecticidal activity of baculovirus insecticides. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Mexico | 1 | 10% |
Unknown | 9 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 40% |
Student > Ph. D. Student | 3 | 30% |
Professor | 1 | 10% |
Student > Master | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 50% |
Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Materials Science | 1 | 10% |