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Baculovirus and Insect Cell Expression Protocols

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Cover of 'Baculovirus and Insect Cell Expression Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior
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    Chapter 2 Introduction to Baculovirus Molecular Biology
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    Chapter 3 Baculovirus Transfer Vectors
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    Chapter 4 Recombinant Baculovirus Isolation
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    Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
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    Chapter 6 Available Lepidopteran Insect Cell Lines.
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    Chapter 7 Lepidopteran Insect Cell Line Isolation from Insect Tissue
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    Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines
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    Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
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    Chapter 10 Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System
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    Chapter 11 Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
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    Chapter 12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
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    Chapter 13 Protein Production with Recombinant Baculoviruses in Lepidopteran Larvae
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    Chapter 14 Production of Virus-Like Particles for Vaccination
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    Chapter 15 Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
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    Chapter 16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
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    Chapter 17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression.
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    Chapter 18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
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    Chapter 19 Introduction to the Use of Baculoviruses as Biological Insecticides
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    Chapter 20 Baculovirus Insecticide Production in Insect Larvae
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    Chapter 21 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
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    Chapter 22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
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    Chapter 23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production
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    Chapter 24 Gene Silencing in Insect Cells Using RNAi
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    Chapter 25 Using the Baculovirus/Insect Cell System to Study Apoptosis
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    Chapter 26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells
Attention for Chapter 15: Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
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Chapter title
Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
Chapter number 15
Book title
Baculovirus and Insect Cell Expression Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3043-2_15
Pubmed ID
Book ISBNs
978-1-4939-3042-5, 978-1-4939-3043-2
Authors

Stephanie Chen, Chen, Stephanie

Abstract

Expression of recombinant proteins in insect cells infected with baculoviruses is commonplace. This system provides an easy way to generate a significant amount of properly folded, functional protein with proper posttranslational modifications and can be used to effectively produce multi-protein complexes. This chapter describes an alternative method of expressing high order protein complexes in insect cell culture. Specific examples involving the expression of 5- and 8-protein complexes are discussed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Student > Ph. D. Student 2 29%
Student > Master 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Agricultural and Biological Sciences 2 29%
Engineering 1 14%
Unknown 1 14%