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Chapter title |
Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
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Chapter number | 15 |
Book title |
Baculovirus and Insect Cell Expression Protocols
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3043-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Stephanie Chen, Chen, Stephanie |
Abstract |
Expression of recombinant proteins in insect cells infected with baculoviruses is commonplace. This system provides an easy way to generate a significant amount of properly folded, functional protein with proper posttranslational modifications and can be used to effectively produce multi-protein complexes. This chapter describes an alternative method of expressing high order protein complexes in insect cell culture. Specific examples involving the expression of 5- and 8-protein complexes are discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 43% |
Student > Ph. D. Student | 2 | 29% |
Student > Master | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 43% |
Agricultural and Biological Sciences | 2 | 29% |
Engineering | 1 | 14% |
Unknown | 1 | 14% |