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Mendeley readers
Chapter title |
Gene Silencing in Insect Cells Using RNAi
|
---|---|
Chapter number | 24 |
Book title |
Baculovirus and Insect Cell Expression Protocols
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Hsuan-Chen Wu, John C. March, William E. Bentley, Wu, Hsuan-Chen, March, John C., Bentley, William E. |
Abstract |
A technique is described for synthesizing and transfecting double stranded RNA (dsRNA) for RNA interference (RNAi) in Sf-21 cell culture. Transfection with dsRNA only requires an hour and the cells usually recover within 12 h. Suggestions for designing dsRNA are included in the methods. Furthermore, websites are provided for rapid and effective dsRNA design. Three kits are essential for using the described methods: RNAqueous(®)-4PCR, Megascript™ T7 kit, and the Superscript™ III kit from Life Technologies, Inc. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 27% |
Student > Doctoral Student | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Student > Master | 2 | 13% |
Professor | 1 | 7% |
Other | 3 | 20% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 40% |
Agricultural and Biological Sciences | 4 | 27% |
Immunology and Microbiology | 1 | 7% |
Engineering | 1 | 7% |
Unknown | 3 | 20% |