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Embryonic Stem Cell Protocols

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Cover of 'Embryonic Stem Cell Protocols'

Table of Contents

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    Book Overview
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    Chapter 126 Osteogenic Differentiation from Embryonic Stem Cells.
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    Chapter 152 Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.
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    Chapter 207 Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium.
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    Chapter 208 From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells.
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    Chapter 209 Generation of Embryonic Stem Cells in Rabbits.
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    Chapter 210 Applying Shear Stress to Pluripotent Stem Cells
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    Chapter 211 A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.
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    Chapter 212 A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
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    Chapter 213 Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool.
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    Chapter 214 Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
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    Chapter 215 Generating Inner Ear Organoids from Mouse Embryonic Stem Cells.
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    Chapter 216 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells
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    Chapter 217 Pancreatic Differentiation from Murine Embryonic Stem Cells
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    Chapter 218 In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production.
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    Chapter 219 Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells
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    Chapter 220 Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells
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    Chapter 221 Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
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    Chapter 226 Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.
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    Chapter 228 Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells.
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    Chapter 229 Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.
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    Chapter 230 In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets.
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    Chapter 231 Generation of Corneal Keratocytes from Human Embryonic Stem Cells.
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    Chapter 232 Embryonic Stem Cell Protocols
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    Chapter 233 Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells.
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    Chapter 234 Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.
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    Chapter 235 Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes
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    Chapter 253 Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition.
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    Chapter 254 Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture.
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    Chapter 255 Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells
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    Chapter 270 Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation.
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    Chapter 271 Embryonic Stem Cell Protocols
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    Chapter 272 Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation
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    Chapter 313 Erratum to: Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture
Attention for Chapter 218: In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production.
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Chapter title
In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production.
Chapter number 218
Book title
Embryonic Stem Cell Protocols
Published in
Methods in molecular biology, July 2015
DOI 10.1007/7651_2015_218
Pubmed ID
Book ISBNs
978-1-4939-2953-5, 978-1-4939-2954-2
Authors

Fauzi, Iliana, Panoskaltsis, Nicki, Mantalaris, Athanasios, Iliana Fauzi, Nicki Panoskaltsis, Athanasios Mantalaris

Abstract

Embryonic stem cells (ESCs) differentiation via embryoid body (EB) formation is an established method that generates the three germ layers. However, EB differentiation poses several problems including formation of heterogeneous cell populations. Herein, we described a differentiation protocol on enhancing mesoderm derivation from murine ESCs (mESCs) using conditioned medium (CM) from HepG2 cells. We used this technique to direct hematopoiesis by generating "embryoid-like" colonies (ELCs) from murine (m) ESCs without following standard formation of EBs. Our CM-mESCs group yielded an almost fivefold increase in ELC formation (p ≤ 0.05) and higher expression of mesoderm genes;-Brachyury-T, Goosecoid, and Flk-1 compared with control mESCs group. Hematopoietic colony formation from CM-mESCs was also enhanced by twofold at days 7 and 14 with earlier colony commitment compared to control mESCs (p ≤ 0.05). This early clonogenic capacity was confirmed morphologically by the presence of nucleated erythrocytes and macrophages as early as day 7 in culture using standard 14-day colony-forming assay. Early expression of hematopoietic primitive (ζ-globin) and definitive (β-globin) erythroid genes and proteins was also observed by day 7 in the CM-treated culture. These data indicate that hematopoietic cells more quickly differentiate from CM-treated, compared with those using standard EB approaches, and provide an efficient bioprocess platform for erythroid-specific differentiation of ESCs.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 23%
Student > Ph. D. Student 2 15%
Student > Master 2 15%
Student > Doctoral Student 1 8%
Unspecified 1 8%
Other 1 8%
Unknown 3 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Agricultural and Biological Sciences 2 15%
Unspecified 1 8%
Medicine and Dentistry 1 8%
Neuroscience 1 8%
Other 1 8%
Unknown 3 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2015.
All research outputs
#15,339,713
of 22,816,807 outputs
Outputs from Methods in molecular biology
#5,340
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Outputs of similar age
#153,968
of 262,950 outputs
Outputs of similar age from Methods in molecular biology
#11
of 25 outputs
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So far Altmetric has tracked 13,121 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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