Chapter title |
Embryonic Stem Cell Protocols
|
---|---|
Chapter number | 232 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, July 2015
|
DOI | 10.1007/7651_2015_232 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2953-5, 978-1-4939-2954-2
|
Authors |
Okada, Atsumasa, Tashiro, Katsuhisa, Yamaguchi, Tomoko, Kawabata, Kenji, Atsumasa Okada, Katsuhisa Tashiro, Tomoko Yamaguchi, Kenji Kawabata |
Abstract |
Flk1-expressing (+) mesodermal cells are useful source for the generation of hematopoietic cells and cardiomyocytes from pluripotent stem cells (PSCs). However, they have been reported as a heterogenous population that includes hematopoietic and cardiac progenitors. Therefore, to provide a method for a highly efficient production of hematopoietic cells and cardiomyocytes, cell surface markers are often used for separating these progenitors in Flk1(+) cells. Our recent study has shown that the expression of coxsackievirus and adenovirus receptor (CAR), a tight junction component molecule, could divide mouse and human PSC- and mouse embryo-derived Flk1(+) cells into Flk1(+)CAR(-) and Flk1(+)CAR(+) cells. Flk1(+)CAR(-) and Flk1(+)CAR(+) cells efficiently differentiated into hematopoietic cells and cardiomyocytes, respectively. These results indicate that CAR is a novel cell surface marker for separating PSC-derived Flk1(+) mesodermal cells into hematopoietic and cardiac progenitors. We herein describe a differentiation method from PSCs into hematopoietic cells and cardiomyocytes based on CAR expression. |
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Members of the public | 2 | 100% |
Mendeley readers
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Demographic breakdown
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Student > Master | 4 | 36% |
Other | 1 | 9% |
Student > Bachelor | 1 | 9% |
Unknown | 1 | 9% |
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Unknown | 2 | 18% |