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Embryonic Stem Cell Protocols

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Cover of 'Embryonic Stem Cell Protocols'

Table of Contents

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    Book Overview
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    Chapter 126 Osteogenic Differentiation from Embryonic Stem Cells.
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    Chapter 152 Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.
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    Chapter 207 Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium.
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    Chapter 208 From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells.
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    Chapter 209 Generation of Embryonic Stem Cells in Rabbits.
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    Chapter 210 Applying Shear Stress to Pluripotent Stem Cells
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    Chapter 211 A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.
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    Chapter 212 A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
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    Chapter 213 Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool.
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    Chapter 214 Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
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    Chapter 215 Generating Inner Ear Organoids from Mouse Embryonic Stem Cells.
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    Chapter 216 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells
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    Chapter 217 Pancreatic Differentiation from Murine Embryonic Stem Cells
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    Chapter 218 In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production.
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    Chapter 219 Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells
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    Chapter 220 Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells
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    Chapter 221 Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
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    Chapter 226 Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.
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    Chapter 228 Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells.
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    Chapter 229 Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.
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    Chapter 230 In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets.
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    Chapter 231 Generation of Corneal Keratocytes from Human Embryonic Stem Cells.
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    Chapter 232 Embryonic Stem Cell Protocols
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    Chapter 233 Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells.
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    Chapter 234 Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.
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    Chapter 235 Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes
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    Chapter 253 Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition.
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    Chapter 254 Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture.
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    Chapter 255 Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells
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    Chapter 270 Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation.
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    Chapter 271 Embryonic Stem Cell Protocols
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    Chapter 272 Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation
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    Chapter 313 Erratum to: Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture
Attention for Chapter 214: Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
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Chapter title
Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
Chapter number 214
Book title
Embryonic Stem Cell Protocols
Published in
Methods in molecular biology, April 2015
DOI 10.1007/7651_2015_214
Pubmed ID
Book ISBNs
978-1-4939-2953-5, 978-1-4939-2954-2
Authors

James Chappell, Ben Boward, Stephen Dalton, Chappell, James, Boward, Ben, Dalton, Stephen

Abstract

The FUCCI indicator system is a powerful tool for spatio-temporal analysis of the cell cycle, but its utility has been restricted so far to a limited range of applications. Here, we describe how to establish and validate the FUCCI system in murine pluripotent stem cells (PSCs) and describe the utility of transgenic FUCCI mice. We then describe how the FUCCI system can be used to generate material for a wide-range of omics-based applications in conjunction with FACS isolation of cells. This significantly broadens the potential applications of FUCCI reporters for studying the molecular basis of development and disease.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 32%
Researcher 5 20%
Student > Bachelor 3 12%
Student > Master 2 8%
Professor 1 4%
Other 0 0%
Unknown 6 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 44%
Agricultural and Biological Sciences 3 12%
Environmental Science 1 4%
Immunology and Microbiology 1 4%
Earth and Planetary Sciences 1 4%
Other 0 0%
Unknown 8 32%