↓ Skip to main content

Embryonic Stem Cell Protocols

Overview of attention for book
Cover of 'Embryonic Stem Cell Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 126 Osteogenic Differentiation from Embryonic Stem Cells.
  3. Altmetric Badge
    Chapter 152 Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.
  4. Altmetric Badge
    Chapter 207 Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium.
  5. Altmetric Badge
    Chapter 208 From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells.
  6. Altmetric Badge
    Chapter 209 Generation of Embryonic Stem Cells in Rabbits.
  7. Altmetric Badge
    Chapter 210 Applying Shear Stress to Pluripotent Stem Cells
  8. Altmetric Badge
    Chapter 211 A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.
  9. Altmetric Badge
    Chapter 212 A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
  10. Altmetric Badge
    Chapter 213 Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool.
  11. Altmetric Badge
    Chapter 214 Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
  12. Altmetric Badge
    Chapter 215 Generating Inner Ear Organoids from Mouse Embryonic Stem Cells.
  13. Altmetric Badge
    Chapter 216 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells
  14. Altmetric Badge
    Chapter 217 Pancreatic Differentiation from Murine Embryonic Stem Cells
  15. Altmetric Badge
    Chapter 218 In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production.
  16. Altmetric Badge
    Chapter 219 Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells
  17. Altmetric Badge
    Chapter 220 Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells
  18. Altmetric Badge
    Chapter 221 Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
  19. Altmetric Badge
    Chapter 226 Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.
  20. Altmetric Badge
    Chapter 228 Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells.
  21. Altmetric Badge
    Chapter 229 Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.
  22. Altmetric Badge
    Chapter 230 In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets.
  23. Altmetric Badge
    Chapter 231 Generation of Corneal Keratocytes from Human Embryonic Stem Cells.
  24. Altmetric Badge
    Chapter 232 Embryonic Stem Cell Protocols
  25. Altmetric Badge
    Chapter 233 Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells.
  26. Altmetric Badge
    Chapter 234 Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.
  27. Altmetric Badge
    Chapter 235 Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes
  28. Altmetric Badge
    Chapter 253 Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition.
  29. Altmetric Badge
    Chapter 254 Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture.
  30. Altmetric Badge
    Chapter 255 Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells
  31. Altmetric Badge
    Chapter 270 Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation.
  32. Altmetric Badge
    Chapter 271 Embryonic Stem Cell Protocols
  33. Altmetric Badge
    Chapter 272 Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation
  34. Altmetric Badge
    Chapter 313 Erratum to: Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture
Attention for Chapter 152: Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

Mentioned by

twitter
4 tweeters

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
13 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.
Chapter number 152
Book title
Embryonic Stem Cell Protocols
Published in
Methods in molecular biology, December 2014
DOI 10.1007/7651_2014_152
Pubmed ID
Book ISBNs
978-1-4939-2953-5, 978-1-4939-2954-2
Authors

Ivan Roubal, Sun Joo Park, Yong Kim, Roubal I, Park SJ, Kim Y, Roubal, Ivan, Park, Sun Joo, Kim, Yong

Abstract

Embryonic stem cells are self-renewing pluripotent cells with competency to differentiate into all three-germ lineages. Many studies have demonstrated the importance of genetic and epigenetic molecular mechanisms in the maintenance of self-renewal and pluripotency. Stem cells are under unique molecular and cellular regulations different from somatic cells. Proper regulation should be ensured to maintain their unique self-renewal and undifferentiated characteristics. Understanding key mechanisms in stem cell biology will be important for the successful application of stem cells for regenerative therapeutic medicine. More importantly practical use of stem cells will require our knowledge on how to properly direct and differentiate stem cells into the necessary type of cells. Embryonic stem cells and adult stem cells have been used as study models to unveil molecular and cellular mechanisms in various signaling pathways. They are especially beneficial to developmental studies where in vivo molecular/cellular study models are not available. We have derived neural stem cells from human embryonic stem cells as a model to study the effect of teratogen in neural development. We have tested commercial neural differentiation system and successfully derived neural precursor cells exhibiting key molecular features of neural stem cells, which will be useful for experimental application.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 23%
Student > Bachelor 3 23%
Student > Master 3 23%
Student > Ph. D. Student 1 8%
Unspecified 1 8%
Other 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Agricultural and Biological Sciences 4 31%
Unspecified 1 8%
Immunology and Microbiology 1 8%
Medicine and Dentistry 1 8%
Other 1 8%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 August 2016.
All research outputs
#13,925,649
of 22,774,233 outputs
Outputs from Methods in molecular biology
#3,919
of 13,091 outputs
Outputs of similar age
#181,611
of 353,309 outputs
Outputs of similar age from Methods in molecular biology
#244
of 983 outputs
Altmetric has tracked 22,774,233 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,091 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,309 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 983 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.