Chapter title |
Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.
|
---|---|
Chapter number | 226 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, March 2015
|
DOI | 10.1007/7651_2015_226 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2953-5, 978-1-4939-2954-2
|
Authors |
Radan, Lida, Hughes, Chris S, Teichroeb, Jonathan H, Postovit, Lynne-Marie, Betts, Dean H, Lida Radan, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, Dean H. Betts, Hughes, Chris S., Teichroeb, Jonathan H., Betts, Dean H. |
Abstract |
Morpholino oligonucleotides (MO) are an innovative tool that provides a means for examining and modifying gene expression outcomes by antisense interaction with targeted RNA transcripts. The site-specific nature of their binding facilitates focused modulation to alter splice variant expression patterns. Here we describe the steric-blocking of human telomerase reverse transcriptase (hTERT) Δα and Δβ splice variants using MO to examine cellular outcomes related to pluripotency and differentiation in human embryonic stem cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Professor | 2 | 18% |
Other | 2 | 18% |
Student > Bachelor | 2 | 18% |
Unspecified | 1 | 9% |
Other | 0 | 0% |
Unknown | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Unspecified | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Immunology and Microbiology | 1 | 9% |
Energy | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |