Chapter title |
Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells
|
---|---|
Chapter number | 255 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, July 2015
|
DOI | 10.1007/7651_2015_255 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2953-5, 978-1-4939-2954-2
|
Authors |
Anna Pezzarossa, Ana M. V. Guedes, Domingos Henrique, Elsa Abranches, Pezzarossa, Anna, Guedes, Ana M. V., Henrique, Domingos, Abranches, Elsa |
Abstract |
The current view of the pluripotent state is that of a transient, dynamic state, maintained by the balance between opposing cues. Understanding how this dynamic state is established in pluripotent cells and how it relates to gene expression is essential to obtain a more detailed description of the pluripotent state.In this chapter, we describe how to study the dynamic expression of a core pluripotency gene regulator-Nanog-by exploiting single-cell time-lapse imaging of a reporter mESC line grown in different cell culture media. We further describe an automated image analysis method and discuss how to extract information from the generated quantitative time-course data. |
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