Chapter title |
Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering
|
---|---|
Chapter number | 21 |
Book title |
Adult Stem Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6756-8_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
Authors |
Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, Syam P. Nukavarapu, Joshi, Shalmli U., Barbu, Rares O., Carr-Reynolds, Melissa, Barnes, Brian, Nukavarapu, Syam P. |
Abstract |
In this chapter, we introduce a completely intraoperative procedure for obtaining a patient-derived biomaterial in cell therapy and tissue engineering applications. An automated device for processing human peripheral-blood ensures a reproducible method for retrieving the patient's cellular-rich as well as cellular-poor plasma. By substituting calcium for animal-derived thrombin, we engineer a completely autologous hydrogel that eliminates the risk of disease transmission and lowers FDA regulation hurdles. Through this chapter, we will discuss a bedside protocol developed to prepare a patient-derived hydrogel. This method can be effectively used to develop a completely intraoperative tissue engineering strategy (CITES) that can be easily translated into the clinic for surgical use. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 17% |
Other | 1 | 17% |
Lecturer | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Other | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 2 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Unknown | 1 | 17% |