Chapter title |
A Simple Protocol to Isolate, Characterize, and Expand Dental Pulp Stem Cells
|
---|---|
Chapter number | 1 |
Book title |
Adult Stem Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6756-8_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
Authors |
Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, Giovanni Nicolao Berta, Di Scipio, Federica, Sprio, Andrea Elio, Carere, Maria Elisabetta, Yang, Zhiqian, Berta, Giovanni Nicolao |
Abstract |
Adult stem cells reside in body tissues to preserve organs and whole organism homeostasis. They are acquiring a prominent role in the contemporary medicine. Many protocols to isolate and cultivate adult stem cells have been so far described, though they are often lengthy, laborious, and require very expansive instruments, materials, and reagents. On this basis, we describe a simple, cheap but at the same time functional method to: (1) isolate dental pulp stem cells (DPSC), (2) expand and cultivate DPSC, (3) cryopreserve DPSC, (4) characterize DPSC, and (5) differentiate DPSC into both mesenchymal and non-mesenchymal lineages. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 17% |
Researcher | 2 | 17% |
Other | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 3 | 25% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Medicine and Dentistry | 1 | 8% |
Unknown | 3 | 25% |