Chapter title |
Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer
|
---|---|
Chapter number | 4 |
Book title |
Adult Stem Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6756-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
Authors |
Giulia Prosdocimo, Mauro Giacca, Prosdocimo, Giulia, Giacca, Mauro |
Abstract |
In contrast to prenatal life, cardiomyocyte proliferation in mammals is rapidly blunted after birth; as a consequence, clinically significant cardiac regeneration does not occur in adulthood. Thus, the modulation of cardiomyocyte proliferation by gene transfer offers an invaluable opportunity to both understand the mechanisms regulating renewal of these cells in the fetus and identify novel strategies for myocardial repair.In this Chapter, we report an exhaustive protocol to isolate, culture, and manipulate the properties of neonatal ventricular rat cardiomyocytes by small RNA transfection or transduction with viral vectors based on the adeno-associated virus, which exhibit exquisite tropism for these cells. We also provide techniques to assess DNA synthesis and cell proliferation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 33% |
Neuroscience | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 2 | 33% |