Chapter title |
Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells
|
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Chapter number | 14 |
Book title |
Adult Stem Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6756-8_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
Authors |
Glen Lester Sequiera, Sekaran Saravanan, Sanjiv Dhingra, Sequiera, Glen Lester, Saravanan, Sekaran, Dhingra, Sanjiv |
Abstract |
This chapter deals with the employment of human-induced pluripotent stem cells (hiPSCs) as a candidate to differentiate into mesenchymal stem cells (MSCs). This would enable to help establish a regular source of human MSCs with the aim of avoiding the problems associated with procuring the MSCs either from different healthy individuals or patients, limited extraction potentials, batch-to-batch variations or from diverse sources such as bone marrow or adipose tissue. The procedures described herein allow for a guided and ensured approach for the regular maintenance of hiPSCs and their subsequent differentiation into MSCs using the prescribed medium. Subsequently, an easy protocol for the successive isolation and purification of the hiPSC-differentiated MSCs is outlined, which is carried out through passaging and can be further sorted through flow cytometry. Further, the maintenance and expansion of the resultant hiPSC-differentiated MSCs using appropriate characterization techniques, i.e., Reverse-transcription PCR and immunostaining is also elaborated. The course of action has been deliberated keeping in mind the awareness and the requisites available to even beginner researchers who mostly have access to regular consumables and medium components found in the general laboratory. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 3 | 19% |
Other | 2 | 13% |
Researcher | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Professor | 1 | 6% |
Other | 3 | 19% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 38% |
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Business, Management and Accounting | 1 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 19% |