Chapter title |
Isolation of Stromal Stem Cells from Adipose Tissue
|
---|---|
Chapter number | 13 |
Book title |
Adult Stem Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6756-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6754-4, 978-1-4939-6756-8
|
Authors |
Maria Prat, Francesca Oltolina, Silvia Antonini, Andrea Zamperone, Prat, Maria, Oltolina, Francesca, Antonini, Silvia, Zamperone, Andrea |
Abstract |
Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium. |
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Demographic breakdown
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Student > Ph. D. Student | 9 | 20% |
Researcher | 6 | 13% |
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Student > Postgraduate | 3 | 7% |
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