Chapter title |
Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.
|
---|---|
Chapter number | 14 |
Book title |
Whole Genome Amplification
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2990-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
Authors |
Dimitriadou, Eftychia, Zamani Esteki, Masoud, Vermeesch, Joris Robert, Eftychia Dimitriadou, Masoud Zamani Esteki, Joris Robert Vermeesch, Esteki, Masoud Zamani |
Abstract |
Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively. |
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