| Chapter title |
Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex ® Single-Cell Whole Genome Amplification Kit (WGA4)
|
|---|---|
| Chapter number | 7 |
| Book title |
Whole Genome Amplification
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2990-0_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
| Authors |
Amin El-Heliebi, Shukun Chen, Thomas Kroneis, El-Heliebi, Amin, Chen, Shukun, Kroneis, Thomas |
| Abstract |
Whole genome amplification (WGA) is a widely used technique allowing multiplying picogram amounts of target DNA by several orders of magnitude. The technique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapter sequences, the DNA is amplified by thermal cycling. This amplification typically yields > 2 mg DNA from a single cell, is suited for amplifying DNA isolated from (partly) degraded samples [e.g. formalin-fixed paraffin-embedded (FFPE) material] and works well when used for array-comparative genome hybridization (array-CGH). |
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