You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Construction of a DNA Library on Microbeads Using Whole Genome Amplification
|
---|---|
Chapter number | 6 |
Book title |
Whole Genome Amplification
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2990-0_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
Authors |
Takaaki Kojima, Bo Zhu, Hideo Nakano, Kojima, Takaaki, Zhu, Bo, Nakano, Hideo |
Abstract |
A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a "bead library", has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 50% |
Student > Master | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 50% |
Engineering | 1 | 50% |