Chapter title |
Whole Genome Amplification from Blood Spot Samples
|
---|---|
Chapter number | 12 |
Book title |
Whole Genome Amplification
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2990-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
Authors |
Karina Meden Sørensen, Sørensen, Karina Meden |
Abstract |
Whole genome amplification is an invaluable technique when working with DNA extracted from blood spots, as the DNA obtained from this source often is too limited for extensive genetic analysis. Two techniques that amplify the entire genome are common. Here, both are described with focus on the benefits and drawbacks of each system. However, in order to obtain the best possible WGA result the quality of input DNA extracted from the blood spot is essential, but also time consumption, flexibility in format and elution volume and price of the technology are factors influencing system choice. Here, three DNA extraction techniques are described and the above aspects are compared between the systems. |
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