| Chapter title |
Principles of Whole-Genome Amplification
|
|---|---|
| Chapter number | 1 |
| Book title |
Whole Genome Amplification
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2990-0_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
| Authors |
Zbigniew Tadeusz Czyz, Stefan Kirsch, Bernhard Polzer, Czyz, Zbigniew Tadeusz, Kirsch, Stefan, Polzer, Bernhard |
| Abstract |
Modern molecular biology relies on large amounts of high-quality genomic DNA. However, in a number of clinical or biological applications this requirement cannot be met, as starting material is either limited (e.g., preimplantation genetic diagnosis (PGD) or analysis of minimal residual cancer) or of insufficient quality (e.g., formalin-fixed paraffin-embedded tissue samples or forensics). As a consequence, in order to obtain sufficient amounts of material to analyze these demanding samples by state-of-the-art modern molecular assays, genomic DNA has to be amplified. This chapter summarizes available technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 39% |
| Student > Master | 5 | 18% |
| Student > Bachelor | 3 | 11% |
| Researcher | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 3 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 43% |
| Agricultural and Biological Sciences | 4 | 14% |
| Medicine and Dentistry | 4 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Computer Science | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 3 | 11% |