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| Chapter title |
Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis
|
|---|---|
| Chapter number | 10 |
| Book title |
Whole Genome Amplification
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2990-0_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2989-4, 978-1-4939-2990-0
|
| Authors |
Thomas Kroneis, Amin El-Heliebi, Kroneis, Thomas, El-Heliebi, Amin |
| Abstract |
This protocol describes the use of a 16plex PCR for the purpose assessing DNA quality after isothermal whole genome amplification (WGA). In short, DNA products, generated by amplification multiple displacement amplification, are forwarded to PCR targeting 15 short tandem repeats (STR) as well as amelogenin generating up to 32 different PCR products. After amplification, the PCR products are separated via capillary electrophoresis and analyzed based on the obtained DNA profiles. Isothermal WGA products of good DNA quality will result in DNA profiles with efficiencies of >90 % of the full DNA profile. |