Chapter title |
Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene Therapy
|
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Chapter number | 17 |
Book title |
Non-Viral Gene Delivery Vectors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3718-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3716-5, 978-1-4939-3718-9
|
Authors |
Junjie Li, Zengshi Zha, Zhishen Ge |
Abstract |
Simultaneous achievement of prolonged retention in blood circulation and efficient gene transfection activity in target tissues has always been a major challenge hindering in vivo applications of nonviral gene vectors via systemic administration. The engineered strategies for efficient systemic gene delivery are under wide investigation. These approaches include the thermo-responsive formation of a hydrophobic intermediate layer on PEG-shielded polyplex micelles. Herein, we constructed novel rod-shaped ternary polyplex micelles (TPMs) via complexation between the mixed block copolymers of poly(ethylene glycol)-b-poly{N'-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} (PEG-b-PAsp(DET)) and poly(N-isopropylacrylamide)-b-PAsp(DET) (PNIPAM-b-PAsp(DET)) and plasmid DNA (pDNA) at room temperature (RT), exhibiting distinct temperature-responsive formation of a hydrophobic intermediate layer between PEG shells and pDNA cores through facile temperature increase from RT to body temperature (~37 °C). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Professor | 2 | 18% |
Student > Ph. D. Student | 2 | 18% |
Student > Doctoral Student | 1 | 9% |
Other | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 27% |
Materials Science | 3 | 27% |
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Chemical Engineering | 1 | 9% |
Unknown | 2 | 18% |