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Mendeley readers
| Chapter title |
Polymer Based Gene Silencing: In Vitro Delivery of SiRNA
|
|---|---|
| Chapter number | 9 |
| Book title |
Non-Viral Gene Delivery Vectors
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3718-9_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3716-5, 978-1-4939-3718-9
|
| Authors |
Margarida I. Simão Carlos, Andreas Schätzlein, Ijeoma Uchegbu |
| Abstract |
Gene silencing may be achieved by harnessing the RNA interference mechanism to effect down-regulation of protein expression. The therapeutic use of siRNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA by N-(2-ethylamino)-6-O-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Other | 2 | 33% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 33% |
| Agricultural and Biological Sciences | 1 | 17% |
| Engineering | 1 | 17% |
| Unknown | 2 | 33% |