| Chapter title |
The Use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus Genus.
|
|---|---|
| Chapter number | 18 |
| Book title |
Pulse Field Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2599-5_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
| Authors |
Gosiewski, Tomasz, Brzychczy-Wloch, Monika, Tomasz Gosiewski, Monika Brzychczy-Wloch |
| Abstract |
Bacteria belonging to the genus Lactobacillus are a group of microorganisms in which there are numerous strains with high probiotic potential. In recent years, interest in probiotics as nutritional supplements and as drugs has significantly increased. In order to carry out research on probiotic bacterial strains, their beneficial effects on the human body should be confirmed and molecular typing should be carried out with the aim of marking a specific strain. Pulse-field gel electrophoresis (PFGE) is the reference method employed for bacterial genotyping, but it is commonly used for typing mainly pathogenic bacteria. The following study describes a way of performing PFGE analysis in genotyping of bacteria species belonging to the genus Lactobacillus: L. fermentum, L. gasseri, L. plantarum and L. rhamnosus. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 33% |
| Other | 2 | 22% |
| Lecturer | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Medicine and Dentistry | 2 | 22% |
| Immunology and Microbiology | 1 | 11% |
| Unknown | 3 | 33% |