Chapter title |
Pulsed-Field Gel Electrophoresis of Salmonella enterica
|
---|---|
Chapter number | 16 |
Book title |
Pulse Field Gel Electrophoresis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2599-5_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
Authors |
Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, Nicola Pugliese, Camarda, Antonio, Circella, Elena, Pupillo, Antonia, Legretto, Marilisa, Marino, Michele, Pugliese, Nicola |
Abstract |
Pulsed-field gel electrophoresis (PFGE) is considered a "gold standard" for the molecular characterization of a number of bacterial strains. Its strength relies on its high discriminatory power, together with its high reproducibility. For many years, an international network, PulseNet International, allows the rapid comparison of PFGE data obtained all over the world, and it provides a valuable tool to promptly recognize the epidemiological dynamics of many pathogens, including Salmonella enterica. Here we describe the laboratory procedure to perform the standardized protocol for the PFGE typing of S. enterica strains. |
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