Chapter title |
PFGE Protocols to Distinguish Subspecies of Lactococcus lactis
|
---|---|
Chapter number | 17 |
Book title |
Pulse Field Gel Electrophoresis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2599-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
Authors |
Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, Paul Ritzenthaler, Bourgeois, Pascal Le, Passerini, Delphine, Coddeville, Michèle, Guellerin, Maéva, Daveran-Mingot, Marie-Line, Ritzenthaler, Paul |
Abstract |
Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction fingerprinting, characterization of plasmid complements, and gene localization by DNA-DNA hybridization of Lactococcus lactis genomes. |
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