Chapter title |
Pulsed-Field Gel Electrophoresis for Leuconostoc mesenteroides and L. pseudomesenteroides
|
---|---|
Chapter number | 19 |
Book title |
Pulse Field Gel Electrophoresis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2599-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
Authors |
Victoria Chuat, Marion Dalmasso, Chuat, Victoria, Dalmasso, Marion |
Abstract |
Pulsed-field gel electrophoresis (PFGE) is a technique using alternating electric fields to help the separation of high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and of applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragment. This method is considered as the "gold standard" for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair. |
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