| Chapter title |
Pulsed Field Gel Electrophoresis for Dairy Propionibacteria
|
|---|---|
| Chapter number | 21 |
| Book title |
Pulse Field Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2599-5_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
| Authors |
Victoria Chuat, Rosangela de Freitas, Marion Dalmasso, Chuat, Victoria, Freitas, Rosangela de, Dalmasso, Marion |
| Abstract |
Pulsed field gel electrophoresis (PFGE) is a technique using alternating electric fields to migrate high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and in applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragments. Among other things, this technique is considered as the "gold standard" for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair. This chapter describes a PFGE method that can be used to differentiate dairy propionibacteria. |
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