Chapter title |
Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam®
|
---|---|
Chapter number | 17 |
Book title |
3D Sponge-Matrix Histoculture
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7745-1_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7743-7, 978-1-4939-7745-1
|
Authors |
Andrea Introini, Wendy Fitzgerald, Christophe Vanpouille, Leonid Margolis |
Abstract |
Gelfoam® histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam® histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam® histoculture at the liquid-air interface. The Gelfoam® histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 33% |
Student > Ph. D. Student | 1 | 11% |
Student > Bachelor | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 44% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Environmental Science | 1 | 11% |
Unknown | 2 | 22% |