Chapter title |
Hair Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair
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Chapter number | 15 |
Book title |
3D Sponge-Matrix Histoculture
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7745-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7743-7, 978-1-4939-7745-1
|
Authors |
Fang Liu, Robert M. Hoffman |
Abstract |
The stem cell marker, nestin, is expressed in the hair follicle, both in cells in the bulge area (BA) and the dermal papilla (DP). Nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells of both the BA and DP have been previously shown to be able to form neurons, heart muscle cells, and other non-follicle cell types. The ability of the nestin-expressing HAP stem cells from the BA and DP to repair spinal cord injury was compared. Nestin-expressing HAP stem cells from both the BA and DP grew very well on Gelfoam®. The HAP stem cells attached to the Gelfoam®within 1 h. They grew along the grids of the Gelfoam®during the first 2 or 3 days. Later they spread into the Gelfoam®. After transplantation of Gelfoam®cultures of nestin-expressing BA or DP HAP stem cells into the injured spinal cord (including the Gelfoam®) nestin-expressing BA and DP cells were observed to be viable over 100 days post-surgery. Hematoxylin and eosin (H&E) staining showed connections between the transplanted cells and the host spine tissue. Immunohistochemistry showed many Tuj1-, Isl 1/2, and EN1-positive cells and nerve fibers in the transplanted area of the spinal cord after BA Gelfoam®or DP Gelfoam®cultures were transplanted to the spine. The spinal cord of mice was injured to effect hind-limb paralysis. Twenty-eight days after transplantation with BA or DP HAP stem cells on Gelfoam®to the injured area of the spine, the mice recovered normal locomotion. |
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