Chapter title |
In Vivo-Like Cell-Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real Time by FUCCI Imaging
|
---|---|
Chapter number | 12 |
Book title |
3D Sponge-Matrix Histoculture
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7745-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7743-7, 978-1-4939-7745-1
|
Authors |
Robert M. Hoffman, Shuya Yano |
Abstract |
FUCCI color codes cells as they express different color fluorescent proteins as they go through phases of the cell cycle. The cell cycle phase distribution of cancer cells in Gelfoam® histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G0/G1. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy in Gelfoam® culture as in vivo; as such, therapy is cell cycle dependent. In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam® histoculture reflects what is observed in vivo. |
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