Chapter title |
Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel
|
---|---|
Chapter number | 19 |
Book title |
3D Sponge-Matrix Histoculture
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7745-1_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7743-7, 978-1-4939-7745-1
|
Authors |
Yasunori Tome, Fuminari Uehara, Fuminori Kanaya, Robert M. Hoffman |
Abstract |
Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to proper gene expression. The flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of avintegrin, and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed 3-dimensional tissue-like structures. These results indicate that Gelfoam®histoculture, unlike Matrigel™ culture, is true 3-dimensional. |
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