Chapter title |
Protective Effects of An Water Extracts Prepared from Loliolus beka Gray Meat Against H 2 O 2 -Induced Oxidative Stress in Chang Liver Cells and Zebrafish Embryo Model
|
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Chapter number | 46 |
Book title |
Taurine 10
|
Published in |
Advances in experimental medicine and biology, January 2017
|
DOI | 10.1007/978-94-024-1079-2_46 |
Pubmed ID | |
Book ISBNs |
978-9-40-241077-8, 978-9-40-241079-2
|
Authors |
Eui Jeong Han, Ju Hyung Um, Eun A. Kim, WonWoo Lee, Nalae Kang, Jae Young Oh, Soo Yeon Park, You-Jin Jeon, Chang-Bum Ahn, Seung Hong Lee, Ginnae Ahn, Han, Eui Jeong, Um, Ju Hyung, Kim, Eun A., Lee, WonWoo, Kang, Nalae, Oh, Jae Young, Park, Soo Yeon, Jeon, You-Jin, Ahn, Chang-Bum, Lee, Seung Hong, Ahn, Ginnae |
Abstract |
In this study, we first evaluated protective effects of Loliolus beka in a human liver cell line and zebrafish embryo model with its anti-oxidant activity. First, we prepared the water extract from L. beka meat (LBMW) at room temperature for 24 h and revealed it consisted of a rich taurine. LBMW exhibited the scavenging effects against 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and hydrogen peroxide (H2O2) as well as the high value of oxygen radical absorbance capacity (ORAC). Also, the hydroxyl radical-induced DNA damage was dose-dependently reduced by the treatment of LBMW. In addition, LBMW showed no cytotoxicity and reduced the production of reactive oxygen species (ROS) in H2O2-treated hepatocytes. Moreover, LBMW regulated the expression of an anti-apoptotic molecule, Bcl-2 and the expression of pro-apoptotic molecules, Bax and PARP in H2O2-treated hepatocytes as well as the increment of antioxidant mediated-HO-1 and Nrf2 protein expression. In further study, LBMW improved the survival rate and decreased the production of ROS in H2O2-treated zebrafish embryo model. Therefore, our results suggest that Loliolus beka has protective effects against H2O2-induced oxidative stress and may be used as a potential source for functional foods. |
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Mendeley readers
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Student > Bachelor | 2 | 20% |
Lecturer | 1 | 10% |
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Unknown | 3 | 30% |
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